500g, Chao Zhi Ke, FRUCTUS AURANTII, Tcm Herbal

English name: FRUCTUS AURANTII
Chinese name: 枳壳
Properties of medicinal materials:
This product has a hemispherical shape with a diameter of 3 to 5 cm. The exocarp is brown or brown, with granular protrusions, and concave oil chambers at the top of the protrusions; there are obvious style remnants or fruit stalk marks. The pericarp of the cut surface is yellow-white, smooth and slightly raised, 0.4～1.3cm thick, with 1～2 rows of oil chambers scattered on the edge, 7～12 petals of the sac, a few to 15 petals, the juice sac shrinks brown to tan, inside Hidden seeds. The quality is hard, not easy to break. It has a fresh fragrance, bitter taste and slightly sour taste.
Processing:
Citrus aurantium: remove impurities, wash, moisturize thoroughly, cut into thin slices, and sift off the broken core after drying. This product
It is an irregular arc-shaped strip, 5cm long and 1.3cm wide. The outer skin of the cut surface is brown to brown, and the mesocarp is yellow-white to yellow-brown. There are 1 to 2 rows of punctate oil chambers near the outer edge, and a few purple-brown sacs on the inner side.
Stir-fried citrus aurantium with bran: take the slices of citrus aurantium and fry according to the bran-fried method (Appendix II D) until the color becomes darker. This product is irregularly curved strip-shaped flakes, darker in color, and some have focal spots.
Character identification:
(1) The powder of this product is yellowish white or brownish yellow. The mesocarp cells are round or irregular in shape, and the walls are mostly unevenly thickened. The surface of the pericarp epidermal cells is polygonal, square-like or rectangular, the stomata are nearly circular, with a diameter of 16-34μm, and 5-9 accessory cells; the outer stratum corneum is viewed from the side. The juice sac tissue is light yellow or colorless, film-like, and the epidermal cells on the surface are elongated, shrunken, and arranged staggered with the underlying cells. Calcium oxalate square crystals are present in the peel and juice sac cells, and are in the shape of a rhombus, polyhedron or biconical shape, with a diameter of 3 to 30 μm. The threads, reticulated ducts and tracheids are small.
(2) Take 0.5g of this product powder, add 10ml of methanol, heat to reflux for 10 minutes, filter, take 1ml of filtrate, add about 5mg of potassium borohydride, shake well, add a few drops of hydrochloric acid, the solution will appear cherry red to purple red.
Function and indication:
Regulate the qi in a broad, stagnation and swelling. It is used for stagnation of qi in the chest and hypothermia, fullness and pain, food accumulation, phlegm stoppage; gastroptosis, prolapse of the anus, prolapse of the ziguan.
Storage of medicinal materials
Store in a cool and dry place to prevent moths.
Cultivation tips:
(1) The main cultivated varieties are Citrus aurantium ‘Huangpi’, Citrus aurantium ‘Daidai’, Citrus aurantium ‘Chuluan’, and Citrus aurantium ‘Tangcheng’.
Assay:
According to high performance liquid chromatography (Appendix VI D) determination.
Chromatographic conditions and system suitability test Use octadecylsilane-bonded silica gel as filler; Acetonitrile-water (21.5:80.5) (adjust pH to 3 with phosphoric acid) as mobile phase;
The detection wavelength is 283nm. The number of theoretical plates should not be less than 2000 based on the naringin peak.
Preparation of reference solution accurately weigh 15mg of naringin reference substance dried under reduced pressure to a constant weight, put it in a 50ml measuring flask, add methanol to the mark, shake up, accurately measure 5ml, place in a 25ml measuring flask, add methanol to Scale, shake well, and get (60μg naringin per 1ml).
Preparation of the test solution: Take about 0.2g of the crude powder of this product, accurately weigh it, and place it in a stoppered conical flask, add 50ml of methanol, close it, weigh it, heat and reflux for 1.5 hours, let it cool, and close it. Then weigh the weight, make up the lost weight with methanol, shake well, filter, accurately measure 10ml of the filtrate, evaporate to dryness, add 3-5ml of water to dissolve the residue, let it cool, and pass through a polyamide column (50-80 mesh, 2g, inner diameter 1.8cm, wet-packed column), eluted with 25ml of water, discarded the aqueous solution, and then eluted with 50ml of methanol. Collect the eluate, transfer to a 50ml measuring flask, add methanol to the mark, shake well, Filter with a microporous membrane (0.45μm), take the filtrate, and get it.
test methods:
Precisely draw 20μl each of the reference solution and the test solution, and inject them into the liquid chromatograph for determination.
This product contains naringin (C27H32O14) not less than 4.0%.

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